Colorado's biopharmaceutical sector thrives on innovation and specialized drug development. Companies in this field focus on a range of therapeutic areas, including oncology, autoimmune disorders, and regenerative medicine. With advanced research capabilities and tight-knit collaborations among stakeholders, the industry is poised for growth. Additionally, the state's vibrant startup ecosystem fosters fresh ideas and technologies, attracting venture capital to support new ventures. This influx of investment and talent paves the way for breakthroughs, positioning Colorado as a key player in the biopharmaceutical domain. For those interested in emerging health solutions, Colorado presents exciting opportunities on the horizon.

The biopharmaceutical firms listed here vary in size and specialization, with most located in Boulder and Golden. Founded between 1990 and 2022, these companies focus on innovative drug developments, ranging from antibody therapies to regenerative treatments. Sizes vary, including small startups with less than 10 employees to larger organizations with hundreds. Collectively, they contribute significantly to oncology, cardiovascular health, and other vital areas. The early-stage ventures demonstrate ambitious goals and novel approaches, reflecting Colorado's rich resource base and research capability in biopharma.

Continue reading to explore the top biopharmaceutical companies in Colorado.

Top 23 Biopharmaceutical Companies in Colorado

1. miRagen Therapeutics, Inc.

• Website: miragen.com
• Ownership type: Private
• Headquarters: Boulder, Colorado, United States (USA)
• Founded year: 2007
• Headcount: 11-50
• LinkedIn: miragen-therapuetics

miRagen Therapeutics, Inc. is a biopharmaceutical company based in Boulder, Colorado, founded in 2007. The company is dedicated to developing innovative medicines aimed at treating autoimmune and rare diseases. With a specialized focus on antibody discovery, miRagen is working on optimized therapeutic candidates, particularly for conditions like Thyroid Eye Disease (TED). Their pipeline includes several investigational therapies currently undergoing clinical trials, reflecting their commitment to improving patient care and treatment outcomes. The company operates with a small team, consisting of 11 to 50 employees, and emphasizes collaboration with the patient community to enhance awareness and support for those affected by the diseases they target.

2. Reven Pharmaceuticals

• Website: reven.com
• Ownership type: Venture Capital
• Headquarters: Golden, Colorado, United States (USA)
• Latest funding: Series D, $23.0M, January 2019
• Founded year: 1999
• Headcount: 11-50
• LinkedIn: reven-pharmaceuticals

Reven Pharmaceuticals, founded in 1999 and based in Golden, Colorado, is a pharmaceutical company dedicated to innovative drug development and therapeutic solutions. The company specializes in addressing unmet medical needs through its proprietary RJX technology, which targets various health conditions, including cancer and cardiovascular diseases. Reven engages in clinical trials and collaborates with research organizations to enhance its offerings. The company has received significant funding, with a Series D round totaling $23 million in 2019, reflecting investor interest in its potential. Reven's mission is to improve patient outcomes by developing advanced treatment options, and it actively seeks to transform the treatment landscape for conditions driven by inflammation and immune dysfunction.

3. Think Bioscience

• Website: thinkbioscience.com
• Ownership type: Venture Capital
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: Seed, $6.0M, May 2024
• Founded year: 2019
• Headcount: 1-10
• LinkedIn: think-bioscience

Think Bioscience, founded in 2019 and based in Boulder, Colorado, is a biotechnology firm dedicated to the development of small-molecule therapeutics. The company leverages synthetic biology to tackle the intricate challenges of drug discovery, particularly for diseases that have few or no effective treatments available. Their innovative platform combines applied microbiology, enzymology, and data science to create new medicines. Think Bioscience has attracted significant investment, including a recent $6 million seed funding round, which underscores the potential of their approach. They collaborate with pharmaceutical companies and research institutions, aiming to provide solutions that enhance drug development processes. Their focus on undruggable targets and complex protein interactions positions them as a relevant player in the biopharmaceutical industry.

4. BioLoomics

• Website: bioloomics.com
• Ownership type: Venture Capital
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: Seed, $8.7M, September 2023
• Founded year: 2019
• Headcount: 11-50
• LinkedIn: bioloomics-llc

BioLoomics, founded in 2019 and based in Boulder, Colorado, is a biotechnology firm dedicated to revolutionizing cancer treatment through the development of advanced antibody-drug conjugates (ADCs). The company leverages cutting-edge techniques in synthetic biology and artificial intelligence to create effective therapies aimed at significant unmet medical needs in oncology. With a team of experts in various scientific disciplines, BioLoomics is focused on discovering antibody-peptide chimeras that can target specific subcellular organelles, enhancing the efficacy and safety of cancer treatments. Recently, they secured $8.7 million in seed funding to further their mission of developing target-degrading antibodies, showcasing their potential for growth and innovation in the biopharmaceutical industry.

5. Millipore Corporation

• Website: emdmillipore.com
• Ownership type: Corporate
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $7.2B, March 2010
• Founded year: 1954
• Headcount: 51-200
• LinkedIn: millipore-corporation

Millipore Corporation, also known as Merck Millipore, is a life science supplier based in Boulder, Colorado. Founded in 1954, the company has established itself as a key provider of products and services tailored for the biotechnology and pharmaceutical sectors. Their extensive portfolio includes analytical tools, biopharmaceutical manufacturing solutions, and water purification systems. These offerings are designed to meet the rigorous demands of research, development, and production processes in drug therapies. Millipore's commitment to quality and compliance with industry standards positions it as a vital partner for organizations seeking reliable materials and solutions in the biopharmaceutical field. The company operates under the umbrella of Merck KGaA, Darmstadt, Germany, and is recognized for its innovative approaches to life science challenges.

6. Mosaic Biosciences

• Website: mosaicbio.com
• Ownership type: Venture Capital
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $11.4M, June 2022
• Founded year: 2009
• Headcount: 11-50
• LinkedIn: mosaic-biosciences

Mosaic Biosciences, based in Boulder, Colorado, is a biopharmaceutical company that specializes in drug discovery and development. Founded in 2009, the company focuses on antibody and protein therapeutics, offering a comprehensive suite of services that help partners navigate the complexities of bringing therapeutic candidates from concept to preclinical stages. With a team of experienced scientists, Mosaic Biosciences employs a data-driven approach and advanced technology to enhance the prospects of clinical success for their partners. They have collaborated with various entities, including start-ups and established biopharmaceutical firms, to advance innovative therapeutic programs. In June 2022, Mosaic secured $11.4 million in funding, which supports their ongoing efforts to expand their drug discovery capabilities and services. Their commitment to quality and collaboration is evident in their partnerships, which aim to solve challenging problems in drug development and optimize therapeutic candidates for clinical use.

7. Aleksia Therapeutics

• Website: aleksiatx.com
• Ownership type: Private
• Headquarters: Golden, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Founded year: 2022
• Headcount: 11-50
• LinkedIn: aleksia-therapeutics

Aleksia Therapeutics, based in Golden, Colorado, is a biotechnology firm founded in 2022. The company is dedicated to developing innovative therapeutic options specifically for cancer patients whose conditions are driven by genetic alterations that conventional therapies cannot effectively address. With a team of experienced scientists and experts, Aleksia employs a scientific approach that combines known biology with innovative chemistry to create better therapeutic options. Their work focuses on covalent drug design, which aims to enhance the selectivity and effectiveness of cancer treatments. Although Aleksia is still in its early stages and has not reported any funding, its commitment to addressing significant medical needs in oncology positions it as a relevant entity in the biopharmaceutical industry.

8. Clovis Oncology

• Website: allos.com
• Ownership type: Corporate
• Headquarters: Westminster, Colorado, United States (USA)
• Latest funding: September 2012
• Founded year: 1992
• Headcount: 51-200
• LinkedIn: allos-therapeutics

Clovis Oncology, also known as Allos, is a pharmaceutical company based in Westminster, Colorado. Founded in 1992, the company specializes in drug development using Causal AI technology. This innovative approach aims to make generics more accessible, particularly in light of the growing needs of an aging population. Allos serves pharmaceutical companies and researchers by optimizing clinical development pathways, making the process faster, cheaper, and safer. Their team consists of experts from various fields, including scientists and pharma professionals, who have worked on over 50 complex drugs. Allos is dedicated to addressing unmet patient needs by delivering safe and effective medications, positioning itself as a significant player in the biopharmaceutical industry.

9. VitriVax

• Website: vitrivaxbio.com
• Ownership type: Venture Capital
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: Other (Grant), $3.6M, December 2024
• Founded year: 2017
• Headcount: 11-50
• LinkedIn: vitrivax

VitriVax is a biotechnology firm based in Boulder, Colorado, established in 2017. The company specializes in vaccine development and formulation technology, aiming to enhance global vaccination efforts. Their focus is on creating innovative solutions, such as thermostable and single-dose vaccines, which are crucial for improving public health outcomes. VitriVax's proprietary ALTA™ technology addresses significant challenges in vaccine distribution, including maintaining stability during transport and reducing the need for multiple doses. This technology allows for long-term storage and ease of transport, making vaccines more accessible, especially in remote areas. The company has received notable funding, including a $3.6 million grant in December 2024, which supports their ongoing research and development initiatives. VitriVax collaborates with healthcare organizations and vaccine manufacturers, contributing to advancements in vaccine technology and public health.

10. Tolmar

• Website: tolmar.com
• Ownership type: Private
• Headquarters: Fort Collins, Colorado, United States (USA)
• Employee distribution: United States (USA) 95%, Canada 3%, Other 2%
• Founded year: 2007
• Headcount: 1001-5000
• LinkedIn: tolmar.

Tolmar is a pharmaceutical company based in Fort Collins, Colorado, founded in 2007. The company specializes in developing innovative treatments primarily in the fields of urology, oncology, and endocrinology. Tolmar is known for its long-acting injectable medications, including Fensolvi®, which treats Central Precocious Puberty, Eligard®, a therapy for advanced prostate cancer, and Jatenzo®, a testosterone replacement therapy. With a workforce of over 900 employees, Tolmar operates a fully integrated business model that includes research and development, manufacturing, and commercialization of its products. The company is dedicated to enhancing patient care and improving quality of life through its specialized treatment offerings. Tolmar's commitment to addressing unmet medical needs and its robust manufacturing capabilities further solidify its role in the biopharmaceutical industry.

11. Greyledge Technologies

• Website: greyledgebiotech.com
• Ownership type: Private
• Headquarters: Lone Tree, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $4.3M, April 2022
• Founded year: 2010
• Headcount: 11-50
• LinkedIn: greyledge-technologies

Greyledge Technologies, founded in 2010 and based in Lone Tree, Colorado, is a clinical biotech company that specializes in regenerative medicine. The company focuses on developing innovative cell therapy products, including Platelet Rich Plasma (PRP) and Bone Marrow Concentrate (BMC), which are utilized by healthcare providers across various medical fields. Their applications span orthopedics, cosmetic surgery, dermatology, and more, showcasing their commitment to enhancing patient care through advanced treatment options. Greyledge employs a unique process that quantifies and controls cell populations within their products, allowing for customized treatments tailored to individual patient needs. The company has received funding amounting to over $4.3 million as of April 2022, indicating investor confidence in their approach and potential for growth. Greyledge's emphasis on data analytics further supports their mission to optimize regenerative medicine practices, making them a notable player in the biopharmaceutical industry.

12. HepQuant, LLC

• Website: hepquant.com
• Ownership type: Private
• Headquarters: Denver, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Founded year: 2007
• Headcount: 11-50
• LinkedIn: hepquant

HepQuant, LLC, based in Denver, Colorado, is a medical diagnostics company founded in 2007. The firm specializes in liver health, developing innovative quantitative tests that provide critical insights into liver function. One of their key products, the HepQuant DuO Test, is a minimally invasive test that measures cholate uptake, a vital indicator of liver health. This test aids healthcare providers in making informed decisions regarding patient management, including the need for invasive procedures and monitoring treatment responses. HepQuant's solutions are not only beneficial for clinicians but are also employed by pharmaceutical companies during clinical trials, enhancing the understanding of liver function and treatment efficacy. The company has collaborated with over 23 pharmaceutical firms and research institutions, supporting various clinical trials related to liver diseases. HepQuant's commitment to improving liver health outcomes through advanced diagnostics positions it as a significant entity in the healthcare industry.

13. RefinedScience

• Website: refinedscience.com
• Ownership type: Private
• Headquarters: Aurora, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Founded year: 2019
• Headcount: 11-50
• LinkedIn: refinedscience

RefinedScience, based in Aurora, Colorado, is a biotechnology firm established in 2019. The company specializes in precision drug development, offering services that include high-fidelity patient data analysis, clinical trial design, and advanced analytics powered by artificial intelligence and machine learning. Their mission is to transform drug discovery and development by providing actionable insights that can significantly increase the probability of success while reducing costs and timelines. RefinedScience has developed a comprehensive platform that integrates curated clinical data and biologic single-cell data, particularly in areas like Acute Myeloid Leukemia (AML). They have built a strong team of experts from various fields, including bioinformatics and molecular biology, to drive their innovative approach. Their collaboration with Argenx to create OncoVerity highlights their commitment to addressing unmet medical needs in oncology. RefinedScience aims to partner with pharmaceutical companies and healthcare organizations to enhance clinical decision-making and optimize drug development processes.

14. Aliri Bioanalysis

• Website: aliribio.com
• Ownership type: Private
• Headquarters: Colorado Springs, Colorado, United States (USA)
• Employee distribution: United States (USA) 77%, France 19%, Other 5%
• Latest funding: July 2008
• Founded year: 1991
• Headcount: 51-200
• LinkedIn: imabiotech

Aliri Bioanalysis, founded in 1991 and based in Colorado Springs, Colorado, is a private bioanalytical contract research organization (CRO) that provides essential services to the pharmaceutical and biotech industries. The company specializes in bioanalysis, method development, and spatial biology, helping clients navigate the intricate processes of drug development. With a team of seasoned scientists and industry experts, Aliri focuses on delivering high-quality data that supports drug programs from discovery through clinical development and regulatory approval. Their commitment to speed and regulatory compliance is evident in their operational practices, which are designed to ensure that clients can file their Investigational New Drug (IND) applications with confidence. Aliri's services are particularly valuable in the current landscape, where the demand for innovative drug development solutions is on the rise. The company has a significant presence in the United States and France, reflecting its international reach and expertise in the field.

15. AJNA BioSciences

• Website: ajnabiosciences.com
• Ownership type: Private
• Headquarters: Littleton, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $2.1M, June 2024
• Founded year: 2021
• Headcount: 11-50
• LinkedIn: ajna-biosciences

AJNA BioSciences, founded in 2021 and based in Littleton, Colorado, is a pharmaceutical company dedicated to the development of botanical drugs. The firm specializes in transforming medicinal plants and fungi into FDA-approved therapies, addressing the increasing demand for safer and more effective treatment options. Their primary focus is on conditions such as Autism Spectrum Disorder and depression, where traditional pharmaceuticals may fall short. AJNA's drug development process is built on a foundation of rigorous research, modern scientific instrumentation, and a commitment to regulatory compliance. The company has recently secured over $2 million in funding, indicating investor confidence in their innovative approach. With a team of experts in drug development, regulatory affairs, and clinical research, AJNA is positioned to make significant contributions to the biopharmaceutical industry, particularly in the emerging field of botanical medicines.

16. Silvergate Pharmaceuticals, Inc.

• Website: silvergatepharma.com
• Ownership type: Corporate
• Headquarters: Greenwood, Colorado, United States (USA)
• Latest funding: June 2019
• Founded year: 2010
• Headcount: 11-50
• LinkedIn: silvergate-pharmaceuticals

Silvergate Pharmaceuticals, Inc., based in Greenwood, Colorado, is a pharmaceutical company dedicated to the development and commercialization of innovative medications tailored for pediatric patients. Founded in 2010, the company has made significant strides in addressing the specific health needs of children, a demographic often overlooked in the pharmaceutical industry. Silvergate's product portfolio includes FDA-approved medications such as Qbrelis, the first oral solution of lisinopril for pediatric patients, and Xatmep, a ready-to-use oral solution of methotrexate. These products are designed to improve accessibility and ease of use for healthcare professionals and patients alike. Silvergate's focus on pediatric drug delivery innovation highlights their commitment to enhancing the quality of care for children, ensuring that safe and effective medications are available to those who need them most.

17. Pyxant Labs

• Website: pyxant.com
• Ownership type: Venture Capital
• Headquarters: Colorado Springs, Colorado, United States (USA)
• Latest funding: $530,000, August 2016
• Founded year: 2000
• Headcount: 51-200
• LinkedIn: pyxant-labs-inc.

Pyxant Labs, now known as Aliri, is a biotechnology firm based in Colorado Springs, Colorado. Founded in 2000, the company has carved out a niche in the biopharmaceutical industry by providing bioanalytical and spatial solutions that are vital for drug development. Aliri serves a range of pharmaceutical and biotech clients, offering services such as bioanalysis, method development, and spatial biology. Their expertise helps clients navigate the complexities of bringing life-saving therapies to market efficiently and in compliance with regulatory standards. Aliri's commitment to innovation and quality data generation is evident in their operational practices, which are designed to support drug development from discovery through clinical phases. The company has received funding, with a notable amount of $530,000 reported in 2016, indicating investor confidence in their business model and growth potential.

18. Corgenix Medical Corporation

• Website: corgenix.com
• Ownership type: Corporate
• Headquarters: Broomfield, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $16.0M, August 2014
• Founded year: 1990
• Headcount: 11-50
• LinkedIn: corgenix-medical-corporation

Corgenix Medical Corporation, founded in 1990 and based in Broomfield, Colorado, is a medical diagnostics company that specializes in providing clinical laboratory services, clinical trial management, and contract development and manufacturing for diagnostic tests. The company operates a CAP/CLIA accredited laboratory and is recognized for its expertise in assay development and regulatory compliance. Corgenix serves a variety of clients, including biotech firms, pharmaceutical companies, cancer centers, and academic laboratories. Their services cover all aspects of the in vitro diagnostic process, from development and quality control to regulatory compliance and commercialization. Corgenix is also a contract development and manufacturing organization (CDMO), offering a platform-agnostic approach to test development, which allows them to cater to the specific needs of their clients. Their commitment to quality is underscored by their ISO-13485 certification and FDA registration, ensuring that they meet stringent regulatory standards. The company has received funding amounting to $16 million as of August 2014, which supports its operations and growth in the diagnostics field.

19. Flagship Biosciences, Inc.

• Website: flagshipbio.com
• Ownership type: Venture Capital
• Headquarters: Broomfield, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: $5.0M, April 2024
• Founded year: 2009
• Headcount: 51-200
• LinkedIn: flagship-biosciences

Flagship Biosciences, Inc., founded in 2009 and based in Broomfield, Colorado, is a biotechnology firm that focuses on spatial biology and biomarker analysis. The company offers a comprehensive suite of services that includes anatomical pathology, genomics, and data science, catering primarily to pharmaceutical and biotechnology clients. Their expertise lies in providing actionable insights that support drug development and clinical trials, particularly in areas such as oncology and rare diseases. Flagship is recognized for its advanced capabilities in digital pathology and image analysis, utilizing proprietary technologies to deliver high-quality, data-rich interpretations of clinical samples. The company is also CLIA-certified and CAP-accredited, underscoring its commitment to quality and regulatory compliance. Recently, Flagship secured $5 million in funding, reflecting continued investor confidence in its innovative approach and growth potential.

20. Mountain View Clinical Research

• Website: mtnresearch.com
• Ownership type: Private
• Headquarters: Denver, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Founded year: 2000
• Headcount: 11-50
• LinkedIn: mountain-view-clinical-research-inc.

Mountain View Clinical Research, founded in 2000 and based in Denver, Colorado, is a clinical research organization dedicated to conducting clinical trials and medical studies across a range of health conditions. With a team of experienced professionals, they focus on ensuring the safety and well-being of study participants while advancing medical knowledge. The organization has been involved in over 100 clinical trials, collaborating with various pharmaceutical companies to explore treatments for conditions such as multiple sclerosis, depression, and anxiety disorders. Their commitment to patient care and ethical research practices is evident in their operations, which adhere to strict regulatory guidelines. Mountain View Clinical Research has established itself as a reliable partner in the biopharmaceutical industry, providing valuable insights and data that support the development of new therapies.

21. PharmaJet

• Website: pharmajet.com
• Ownership type: Venture Capital
• Headquarters: Golden, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Latest funding: October 2023
• Founded year: 2005
• Headcount: 11-50
• LinkedIn: pharmajet-inc

PharmaJet, founded in 2005 and based in Golden, Colorado, is a commercial stage MedTech company that specializes in needle-free injection systems designed for vaccines and therapeutics. Their innovative products, such as the Tropis and Stratis systems, aim to enhance vaccine delivery, improve immunogenicity, and provide a more comfortable experience for patients. PharmaJet's technology has been validated through various regulatory approvals, including CE Mark and WHO PQS certification, demonstrating their commitment to safety and efficacy. The company collaborates with pharmaceutical firms and healthcare organizations to increase vaccination coverage and reduce the risks associated with traditional needle injections. Their systems are particularly relevant in the context of global health challenges, including the COVID-19 pandemic, where effective and safe vaccine delivery is crucial. With a focus on improving access to life-saving pharmaceuticals, PharmaJet is actively involved in advancing the field of biopharmaceuticals.

22. Cerapedics Inc.

• Website: cerapedics.com
• Ownership type: Venture Capital
• Headquarters: Westminster, Colorado, United States (USA)
• Employee distribution: United States (USA) 96%, Other 4%
• Latest funding: $25.0M, March 2022
• Founded year: 2001
• Headcount: 201-500
• LinkedIn: cerapedics

Cerapedics Inc., founded in 2001 and headquartered in Westminster, Colorado, is a commercial-stage company that specializes in ortho-biologics. The company focuses on developing biologic bone grafts, with its primary product, i-FACTOR, being a biologic bone graft that combines a small peptide (P-15) with an anorganic bone mineral. This innovative product is designed to enhance the natural bone healing process, making it a valuable tool for healthcare providers and surgeons in orthopedic and trauma fields. Cerapedics has received significant funding, with its last funding round amounting to nearly $25 million in March 2022. The company operates globally and has been involved in clinical studies to further validate the efficacy of its products, including a pivotal IDE study for lumbar interbody fusion. Cerapedics aims to transform the standard of care for bone repair, striving to improve patient outcomes through its advanced biologic solutions.

23. Darwin Biosciences

• Website: darwin.bio
• Ownership type: Private
• Headquarters: Boulder, Colorado, United States (USA)
• Employee distribution: United States (USA) 100%
• Founded year: 2020
• Headcount: 11-50
• LinkedIn: darwin-biosciences

Darwin Biosciences, founded in 2020 and based in Boulder, Colorado, is a private biotechnology firm dedicated to developing innovative diagnostic solutions for infectious diseases. The company specializes in saliva-based diagnostics, which enable early detection of infections, a critical capability in managing public health. Their technology leverages signals from human saliva to identify infections before symptoms appear, thus allowing for timely interventions. Darwin's initial focus was on creating a testing solution for SARS-CoV-2 during the COVID-19 pandemic, which propelled their growth and established their expertise in the field. They are now working on a next-generation diagnostic platform that aims to prepare for future pathogen threats. Collaborating with institutions like the University of Colorado Boulder, Darwin benefits from advanced research and resources, enhancing their product development efforts. Their commitment to innovation and public health is evident in their partnerships with various healthcare organizations and government entities, positioning them as a significant player in the biopharmaceutical industry.

Biopharmaceutical Insights: Key Companies in Colorado

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